Pfu DNA Polymerase is a thermostable enzyme with a molecular weight of 90 kDa. It catalyzes the polymerization of nucleotides into duplex DNA in the 5′_3′ direction, resulting in blunt-ended PCR products without 3′-dA overhangs.
Pfu DNA Polymerase exhibits 3′_5′ exonuclease (proofreading) activity that enables the polymerase to correct the mis-incorporation of nucleotide, and lacks 5′_3′ exonuclease activity. It is suitable for PCR and primer extension reaction that requires high fidelity when the PCR fragment is relatively shorter than 3kb.

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