DNA Green Viewer™ is a new and safe nucleic acid stain, an alternative to the traditional ethidium bromide (EB) stain for detecting double-stranded DNA, single-stranded DNA, and RNA in agarose gels. It emits either green fluorescence when bound to dsDNA or red fluorescence when bound to ssDNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at approximately 290 nm and one at approximately 490 nm. DNA Green Viewer™ is as sensitive as EB. The staining protocol for DNA Green Viewer™ is similar to that for EB. Compared to EB, known as a strong mutagen, DNA Green Viewer™ causes much fewer mutations in the Ames test. In contrast, DNA Green Viewer™ has a negative test in mouse marrow chromophilous erythrocyte micronucleus result and mouse spermary spermatocyte chromosomal aberration test. So it is wise to choose DNA Green Viewer™ instead of EB for detecting nucleic acid in agarose gels.

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