This DNA polymerase is a mixture of Taq DNA polymerase and a temperature sensitive, aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 40°C, but releases the enzyme during normal PCR cycling conditions. The aptamer-based hot start mechanism does not require a separate high temperature incubation step to activate the enzyme. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification.

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